Platinum in PDB 2aeo: Crystal Structure of Cisplatinated Bovine Cu,Zn Superoxide Dismutase

All present enzymatic activity of Crystal Structure of Cisplatinated Bovine Cu,Zn Superoxide Dismutase:
1.15.1.1;

The structure of Crystal Structure of Cisplatinated Bovine Cu,Zn Superoxide Dismutase, PDB code: 2aeo was solved by V.Calderone, A.Casini, S.Mangani, L.Messori, P.L.Orioli, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	44.90 / 1.80
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	47.207, 50.920, 146.738, 90.00, 90.00, 90.00
R / Rfree (%)	20.2 / 23.6

The structure of Crystal Structure of Cisplatinated Bovine Cu,Zn Superoxide Dismutase also contains other interesting chemical elements:

Copper	(Cu)	2 atoms
Chlorine	(Cl)	4 atoms
Zinc	(Zn)	2 atoms

The binding sites of Platinum atom in the Crystal Structure of Cisplatinated Bovine Cu,Zn Superoxide Dismutase (pdb code 2aeo). This binding sites where shown within 5.0 Angstroms radius around Platinum atom.
In total 2 binding sites of Platinum where determined in the Crystal Structure of Cisplatinated Bovine Cu,Zn Superoxide Dismutase, PDB code: 2aeo:
Jump to Platinum binding site number: 1; 2;

Platinum binding site 1 out of 2 in the Crystal Structure of Cisplatinated Bovine Cu,Zn Superoxide Dismutase


Mono view


Stereo pair view

A full contact list of Platinum with other atoms in the Pt binding site number 1 of Crystal Structure of Cisplatinated Bovine Cu,Zn Superoxide Dismutase within 5.0Å range: probe atom residue distance (Å) B Occ A:Pt284 b:16.1 occ:0.70 PT1 A:CPT284 0.0 16.1 0.7 NE2 A:HIS19 2.2 13.5 1.0 CL2 A:CPT284 2.3 11.6 0.7 CL1 A:CPT284 2.5 26.0 0.7 CD2 A:HIS19 3.2 11.2 1.0 CE1 A:HIS19 3.2 11.9 1.0 O A:HOH428 3.5 14.0 1.0 CG2 A:THR17 4.0 6.5 1.0 CB A:THR17 4.3 6.5 1.0 CG A:HIS19 4.3 8.3 1.0 ND1 A:HIS19 4.3 11.5 1.0 O A:HOH424 4.7 27.2 1.0 OG1 A:THR17 5.0 7.7 1.0 O A:HOH388 5.0 9.2 1.0

Platinum binding site 2 out of 2 in the Crystal Structure of Cisplatinated Bovine Cu,Zn Superoxide Dismutase


Mono view


Stereo pair view

A full contact list of Platinum with other atoms in the Pt binding site number 2 of Crystal Structure of Cisplatinated Bovine Cu,Zn Superoxide Dismutase within 5.0Å range: probe atom residue distance (Å) B Occ B:Pt287 b:13.4 occ:0.60 PT1 B:CPT287 0.0 13.4 0.6 NE2 B:HIS19 2.2 10.4 1.0 CL1 B:CPT287 2.4 7.7 0.6 CL2 B:CPT287 2.5 14.4 0.6 CD2 B:HIS19 3.1 10.3 1.0 CE1 B:HIS19 3.2 9.3 1.0 OG1 B:THR17 3.9 10.5 1.0 CG B:HIS19 4.2 8.1 1.0 O B:HOH339 4.3 18.9 1.0 ND1 B:HIS19 4.3 10.7 1.0 O B:HOH350 4.4 5.8 1.0 CG2 B:THR17 5.0 7.7 1.0 CB B:THR17 5.0 7.8 1.0

V.Calderone, A.Casini, S.Mangani, L.Messori, P.L.Orioli. Structural Investigation of Cisplatin-Protein Interactions: Selective Platination of HIS19 in A Cuprozinc Superoxide Dismutase. Angew. Chem. Int. Ed. Engl. V. 45 1267 2006.
ISSN: ISSN 1433-7851
PubMed: 16416478
DOI: 10.1002/ANIE.200502599