Platinum in PDB 4gcf: Room Temperature X-Ray Diffraction Study of A 6-Fold Molar Excess of A Cisplatin/Carboplatin Mixture Binding to Hewl, Dataset 4

All present enzymatic activity of Room Temperature X-Ray Diffraction Study of A 6-Fold Molar Excess of A Cisplatin/Carboplatin Mixture Binding to Hewl, Dataset 4:
3.2.1.17;

The structure of Room Temperature X-Ray Diffraction Study of A 6-Fold Molar Excess of A Cisplatin/Carboplatin Mixture Binding to Hewl, Dataset 4, PDB code: 4gcf was solved by J.R.Helliwell, S.W.M.Tanley, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.74 / 3.50
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	79.870, 79.870, 37.768, 90.00, 90.00, 90.00
R / Rfree (%)	17 / 23.5

The binding sites of Platinum atom in the Room Temperature X-Ray Diffraction Study of A 6-Fold Molar Excess of A Cisplatin/Carboplatin Mixture Binding to Hewl, Dataset 4 (pdb code 4gcf). This binding sites where shown within 5.0 Angstroms radius around Platinum atom.
In total 2 binding sites of Platinum where determined in the Room Temperature X-Ray Diffraction Study of A 6-Fold Molar Excess of A Cisplatin/Carboplatin Mixture Binding to Hewl, Dataset 4, PDB code: 4gcf:
Jump to Platinum binding site number: 1; 2;

Platinum binding site 1 out of 2 in the Room Temperature X-Ray Diffraction Study of A 6-Fold Molar Excess of A Cisplatin/Carboplatin Mixture Binding to Hewl, Dataset 4


Mono view


Stereo pair view

A full contact list of Platinum with other atoms in the Pt binding site number 1 of Room Temperature X-Ray Diffraction Study of A 6-Fold Molar Excess of A Cisplatin/Carboplatin Mixture Binding to Hewl, Dataset 4 within 5.0Å range: probe atom residue distance (Å) B Occ A:Pt201 b:86.8 occ:0.59 NE2 A:HIS15 2.4 77.7 1.0 CE1 A:HIS15 3.0 76.7 1.0 NH1 A:ARG14 3.1 76.1 1.0 CD2 A:HIS15 3.4 74.8 1.0 ND1 A:HIS15 4.2 75.3 1.0 CZ A:ARG14 4.4 79.0 1.0 OD2 A:ASP87 4.4 73.2 1.0 CG A:HIS15 4.4 71.6 1.0 CG A:ASP87 4.9 69.4 1.0 OG1 A:THR89 4.9 61.9 1.0 CB A:ALA11 5.0 50.0 1.0

Platinum binding site 2 out of 2 in the Room Temperature X-Ray Diffraction Study of A 6-Fold Molar Excess of A Cisplatin/Carboplatin Mixture Binding to Hewl, Dataset 4


Mono view


Stereo pair view

A full contact list of Platinum with other atoms in the Pt binding site number 2 of Room Temperature X-Ray Diffraction Study of A 6-Fold Molar Excess of A Cisplatin/Carboplatin Mixture Binding to Hewl, Dataset 4 within 5.0Å range: probe atom residue distance (Å) B Occ A:Pt202 b:0.2 occ:0.71 ND1 A:HIS15 2.8 75.3 1.0 CG A:HIS15 3.6 71.6 1.0 CB A:HIS15 3.7 65.8 1.0 CE1 A:HIS15 3.8 76.7 1.0 CA A:HIS15 3.8 65.6 1.0 O A:HIS15 4.1 63.9 1.0 OD1 A:ASN93 4.3 65.9 1.0 CG2 A:THR89 4.3 61.4 1.0 C A:HIS15 4.4 63.5 1.0 OG1 A:THR89 4.8 61.9 1.0 CD2 A:HIS15 4.8 74.8 1.0 NE2 A:HIS15 4.8 77.7 1.0

J.R.Helliwell, S.W.Tanley. The Crystal Structure Analysis of the Relative Binding of Cisplatin and Carboplatin in A Mixture with Histidine in A Protein Studied at 100 and 300 K with Repeated X-Ray Irradiation. Acta Crystallogr.,Sect.D V. 69 121 2013.
ISSN: ISSN 0907-4449
PubMed: 23275170
DOI: 10.1107/S090744491204423X