Platinum in PDB 4lt3: Hewl Co-Crystallized with Carboplatin in Non-Nacl Conditions: Crystal 2 Processed Using the Xds Software Package

All present enzymatic activity of Hewl Co-Crystallized with Carboplatin in Non-Nacl Conditions: Crystal 2 Processed Using the Xds Software Package:
3.2.1.17;

The structure of Hewl Co-Crystallized with Carboplatin in Non-Nacl Conditions: Crystal 2 Processed Using the Xds Software Package, PDB code: 4lt3 was solved by S.W.M.Tanley, K.Diederichs, L.M.J.Kroon-Batenburg, A.M.M.Schreurs, J.R.Helliwell, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	27.28 / 2.00
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	77.120, 77.120, 36.560, 90.00, 90.00, 90.00
R / Rfree (%)	19.6 / 25.8

The binding sites of Platinum atom in the Hewl Co-Crystallized with Carboplatin in Non-Nacl Conditions: Crystal 2 Processed Using the Xds Software Package (pdb code 4lt3). This binding sites where shown within 5.0 Angstroms radius around Platinum atom.
In total 2 binding sites of Platinum where determined in the Hewl Co-Crystallized with Carboplatin in Non-Nacl Conditions: Crystal 2 Processed Using the Xds Software Package, PDB code: 4lt3:
Jump to Platinum binding site number: 1; 2;

Platinum binding site 1 out of 2 in the Hewl Co-Crystallized with Carboplatin in Non-Nacl Conditions: Crystal 2 Processed Using the Xds Software Package


Mono view


Stereo pair view

A full contact list of Platinum with other atoms in the Pt binding site number 1 of Hewl Co-Crystallized with Carboplatin in Non-Nacl Conditions: Crystal 2 Processed Using the Xds Software Package within 5.0Å range: probe atom residue distance (Å) B Occ A:Pt207 b:51.0 occ:0.23 PT1 A:QPT207 0.0 51.0 0.2 O1 A:QPT207 2.0 53.9 1.0 ND1 A:HIS15 2.7 28.4 1.0 C1 A:QPT207 3.0 49.4 1.0 CE1 A:HIS15 3.6 27.3 1.0 CG A:HIS15 3.7 28.0 1.0 CA A:HIS15 3.8 28.0 1.0 CB A:HIS15 3.9 29.3 1.0 O A:HIS15 4.0 30.9 1.0 OD1 A:ASN93 4.2 40.8 1.0 C A:HIS15 4.4 28.4 1.0 CG2 A:THR89 4.5 24.8 1.0 NE2 A:HIS15 4.8 31.1 1.0 CD2 A:HIS15 4.8 31.1 1.0 ND2 A:ASN93 4.8 38.5 1.0 CG A:ASN93 4.9 33.6 1.0 O A:ARG14 4.9 23.5 1.0 N A:HIS15 5.0 28.0 1.0

Platinum binding site 2 out of 2 in the Hewl Co-Crystallized with Carboplatin in Non-Nacl Conditions: Crystal 2 Processed Using the Xds Software Package


Mono view


Stereo pair view

A full contact list of Platinum with other atoms in the Pt binding site number 2 of Hewl Co-Crystallized with Carboplatin in Non-Nacl Conditions: Crystal 2 Processed Using the Xds Software Package within 5.0Å range: probe atom residue distance (Å) B Occ A:Pt208 b:86.6 occ:0.38 PT1 A:QPT208 0.0 86.6 0.4 O1 A:QPT208 2.0 69.4 1.0 O2 A:QPT208 2.0 61.7 1.0 NE2 A:HIS15 2.8 31.1 1.0 C1 A:QPT208 3.0 66.3 1.0 C2 A:QPT208 3.0 63.0 1.0 NH1 A:ARG14 3.5 49.8 1.0 OD1 A:ASP87 3.6 32.7 1.0 CE1 A:HIS15 3.7 27.3 1.0 CD2 A:HIS15 3.8 31.1 1.0 N A:ILE88 3.9 25.4 1.0 OG1 A:THR89 4.1 30.5 1.0 CG1 A:ILE88 4.1 23.5 1.0 O4 A:QPT208 4.2 64.5 1.0 CG A:ASP87 4.3 31.9 1.0 CA A:ASP87 4.4 25.4 1.0 O A:HOH336 4.5 46.2 1.0 C A:ASP87 4.6 27.0 1.0 CB A:ASP87 4.7 27.4 1.0 N A:THR89 4.7 22.4 1.0 CZ A:ARG14 4.8 52.1 1.0 ND1 A:HIS15 4.9 28.4 1.0 CA A:ILE88 4.9 24.9 1.0 CB A:ALA11 4.9 24.4 1.0 CG A:HIS15 5.0 28.0 1.0

S.W.Tanley, K.Diederichs, L.M.Kroon-Batenburg, C.Levy, A.M.Schreurs, J.R.Helliwell. Carboplatin Binding to Histidine. Acta Crystallogr.,Sect.F V. 70 1135 2014.
ISSN: ISSN 1744-3091
PubMed: 25195881
DOI: 10.1107/S2053230X14016161