Platinum in PDB 4nsg: Carboplatin Binding to Hewl in Nabr Crystallisation Conditions Studied at An X-Ray Wavelength of 1.5418A

All present enzymatic activity of Carboplatin Binding to Hewl in Nabr Crystallisation Conditions Studied at An X-Ray Wavelength of 1.5418A:
3.2.1.17;

The structure of Carboplatin Binding to Hewl in Nabr Crystallisation Conditions Studied at An X-Ray Wavelength of 1.5418A, PDB code: 4nsg was solved by S.W.M.Tanley, K.Diederichs, L.M.J.Kroon-Batenburg, C.Levy, A.M.M.Schreurs, J.R.Helliwell, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	55.42 / 2.00
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	78.377, 78.377, 37.213, 90.00, 90.00, 90.00
R / Rfree (%)	21.9 / 27.1

The structure of Carboplatin Binding to Hewl in Nabr Crystallisation Conditions Studied at An X-Ray Wavelength of 1.5418A also contains other interesting chemical elements:

Bromine	(Br)	9 atoms
Sodium	(Na)	1 atom

The binding sites of Platinum atom in the Carboplatin Binding to Hewl in Nabr Crystallisation Conditions Studied at An X-Ray Wavelength of 1.5418A (pdb code 4nsg). This binding sites where shown within 5.0 Angstroms radius around Platinum atom.
In total 2 binding sites of Platinum where determined in the Carboplatin Binding to Hewl in Nabr Crystallisation Conditions Studied at An X-Ray Wavelength of 1.5418A, PDB code: 4nsg:
Jump to Platinum binding site number: 1; 2;

Platinum binding site 1 out of 2 in the Carboplatin Binding to Hewl in Nabr Crystallisation Conditions Studied at An X-Ray Wavelength of 1.5418A


Mono view


Stereo pair view

A full contact list of Platinum with other atoms in the Pt binding site number 1 of Carboplatin Binding to Hewl in Nabr Crystallisation Conditions Studied at An X-Ray Wavelength of 1.5418A within 5.0Å range: probe atom residue distance (Å) B Occ A:Pt220 b:18.4 occ:0.94 ND1 A:HIS15 2.2 16.1 1.0 BR A:BR204 2.5 22.1 0.9 BR A:BR203 2.5 18.5 0.9 CE1 A:HIS15 3.1 16.0 1.0 CG A:HIS15 3.1 16.0 1.0 CB A:HIS15 3.4 16.0 1.0 CA A:HIS15 3.5 16.0 1.0 O A:HOH303 4.1 22.4 1.0 NE2 A:HIS15 4.2 15.9 1.0 CD2 A:HIS15 4.2 15.9 1.0 O A:HIS15 4.2 16.4 1.0 C A:HIS15 4.3 16.2 1.0 N A:HIS15 4.6 15.8 1.0 CG2 A:THR89 4.7 12.5 1.0 O A:ARG14 4.8 15.8 1.0 OG1 A:THR89 4.8 12.6 1.0

Platinum binding site 2 out of 2 in the Carboplatin Binding to Hewl in Nabr Crystallisation Conditions Studied at An X-Ray Wavelength of 1.5418A


Mono view


Stereo pair view

A full contact list of Platinum with other atoms in the Pt binding site number 2 of Carboplatin Binding to Hewl in Nabr Crystallisation Conditions Studied at An X-Ray Wavelength of 1.5418A within 5.0Å range: probe atom residue distance (Å) B Occ A:Pt221 b:43.3 occ:0.28 NH1 A:ARG14 2.1 17.8 1.0 BR A:BR201 2.3 11.3 0.4 NE2 A:HIS15 2.7 15.9 1.0 CZ A:ARG14 3.3 17.5 1.0 CE1 A:HIS15 3.5 16.0 1.0 CD2 A:HIS15 3.8 15.9 1.0 O A:HOH369 3.8 28.7 1.0 NH2 A:ARG14 3.9 17.9 1.0 NE A:ARG14 4.2 17.1 1.0 OD2 A:ASP87 4.3 13.8 1.0 OD1 A:ASP87 4.3 13.5 1.0 CD A:ARG14 4.4 16.7 1.0 CG A:ASP87 4.4 13.4 1.0 ND1 A:HIS15 4.7 16.1 1.0 OG1 A:THR89 4.8 12.6 1.0 CG A:HIS15 4.8 16.0 1.0 N A:ILE88 5.0 12.7 1.0 CG1 A:ILE88 5.0 12.7 1.0 CB A:ALA11 5.0 12.0 1.0

S.W.Tanley, K.Diederichs, L.M.Kroon-Batenburg, C.Levy, A.M.Schreurs, J.R.Helliwell. Carboplatin Binding to Histidine. Acta Crystallogr F Struct V. 70 1135 2014BIOL Commun.
PubMed: 25195881
DOI: 10.1107/S2053230X14016161