Platinum in PDB 4nsi: Carboplatin Binding to Hewl in 20% Propanol, 20% Peg 4000 at PH5.6

All present enzymatic activity of Carboplatin Binding to Hewl in 20% Propanol, 20% Peg 4000 at PH5.6:
3.2.1.17;

The structure of Carboplatin Binding to Hewl in 20% Propanol, 20% Peg 4000 at PH5.6, PDB code: 4nsi was solved by S.W.M.Tanley, K.Diederichs, L.M.J.Kroon-Batenburg, C.Levy, A.M.M.Schreurs, J.R.Helliwell, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	33.31 / 2.30
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	77.713, 77.713, 36.840, 90.00, 90.00, 90.00
R / Rfree (%)	22 / 28.5

The binding sites of Platinum atom in the Carboplatin Binding to Hewl in 20% Propanol, 20% Peg 4000 at PH5.6 (pdb code 4nsi). This binding sites where shown within 5.0 Angstroms radius around Platinum atom.
In total 2 binding sites of Platinum where determined in the Carboplatin Binding to Hewl in 20% Propanol, 20% Peg 4000 at PH5.6, PDB code: 4nsi:
Jump to Platinum binding site number: 1; 2;

Platinum binding site 1 out of 2 in the Carboplatin Binding to Hewl in 20% Propanol, 20% Peg 4000 at PH5.6


Mono view


Stereo pair view

A full contact list of Platinum with other atoms in the Pt binding site number 1 of Carboplatin Binding to Hewl in 20% Propanol, 20% Peg 4000 at PH5.6 within 5.0Å range: probe atom residue distance (Å) B Occ A:Pt204 b:52.6 occ:0.46 NH2 A:ARG14 2.5 53.1 1.0 NE2 A:HIS15 2.6 31.6 1.0 CE1 A:HIS15 3.1 31.2 1.0 CZ A:ARG14 3.4 49.4 1.0 NE A:ARG14 3.5 49.0 1.0 CD2 A:HIS15 3.8 33.1 1.0 OD1 A:ASP87 4.0 37.5 1.0 ND1 A:HIS15 4.4 31.1 1.0 OG1 A:THR89 4.4 29.8 1.0 O A:HOH327 4.5 28.9 1.0 NH1 A:ARG14 4.6 48.5 1.0 CG1 A:ILE88 4.7 29.7 1.0 CG A:ASP87 4.7 34.5 1.0 CG A:HIS15 4.7 33.1 1.0 CB A:ALA11 4.8 26.6 1.0 O A:HOH314 4.8 38.6 1.0 N A:ILE88 4.8 29.9 1.0 CD A:ARG14 4.9 47.8 1.0

Platinum binding site 2 out of 2 in the Carboplatin Binding to Hewl in 20% Propanol, 20% Peg 4000 at PH5.6


Mono view


Stereo pair view

A full contact list of Platinum with other atoms in the Pt binding site number 2 of Carboplatin Binding to Hewl in 20% Propanol, 20% Peg 4000 at PH5.6 within 5.0Å range: probe atom residue distance (Å) B Occ A:Pt205 b:55.8 occ:0.65 PT1 A:QPT205 0.0 55.8 0.7 O1 A:QPT205 2.0 45.5 1.0 O2 A:QPT205 2.0 56.6 1.0 N2 A:QPT205 2.0 55.2 1.0 ND1 A:HIS15 2.5 31.1 1.0 C1 A:QPT205 2.9 42.4 1.0 C2 A:QPT205 3.0 55.0 1.0 CG A:HIS15 3.4 33.1 1.0 CE1 A:HIS15 3.5 31.2 1.0 CA A:HIS15 3.5 34.8 1.0 CB A:HIS15 3.6 34.1 1.0 O4 A:QPT205 3.9 40.6 1.0 O3 A:QPT205 4.2 56.3 1.0 O A:HIS15 4.2 32.1 1.0 C A:HIS15 4.3 34.4 1.0 O A:ARG14 4.3 35.3 1.0 N A:HIS15 4.6 36.0 1.0 CD2 A:HIS15 4.6 33.1 1.0 NE2 A:HIS15 4.6 31.6 1.0 OG1 A:THR89 4.6 29.8 1.0 C A:ARG14 4.8 36.7 1.0 NZ A:LYS96 4.9 29.4 1.0

S.W.Tanley, K.Diederichs, L.M.Kroon-Batenburg, C.Levy, A.M.Schreurs, J.R.Helliwell. Carboplatin Binding to Histidine. Acta Crystallogr F Struct V. 70 1135 2014BIOL Commun.
PubMed: 25195881
DOI: 10.1107/S2053230X14016161