Platinum in PDB 5fcp: X-Ray Structure of the Adduct Between Hen Egg White Lysozyme and Cisplatin at Long Incubation Times

All present enzymatic activity of X-Ray Structure of the Adduct Between Hen Egg White Lysozyme and Cisplatin at Long Incubation Times:
3.2.1.17;

The structure of X-Ray Structure of the Adduct Between Hen Egg White Lysozyme and Cisplatin at Long Incubation Times, PDB code: 5fcp was solved by I.Russo Krauss, G.Ferraro, A.Pica, A.Merlino, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	56.06 / 1.55
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	79.280, 79.280, 37.450, 90.00, 90.00, 90.00
R / Rfree (%)	16.8 / 21.3

The structure of X-Ray Structure of the Adduct Between Hen Egg White Lysozyme and Cisplatin at Long Incubation Times also contains other interesting chemical elements:

Chlorine

(Cl)

3 atoms

The binding sites of Platinum atom in the X-Ray Structure of the Adduct Between Hen Egg White Lysozyme and Cisplatin at Long Incubation Times (pdb code 5fcp). This binding sites where shown within 5.0 Angstroms radius around Platinum atom.
In total 2 binding sites of Platinum where determined in the X-Ray Structure of the Adduct Between Hen Egg White Lysozyme and Cisplatin at Long Incubation Times, PDB code: 5fcp:
Jump to Platinum binding site number: 1; 2;

Platinum binding site 1 out of 2 in the X-Ray Structure of the Adduct Between Hen Egg White Lysozyme and Cisplatin at Long Incubation Times


Mono view


Stereo pair view

A full contact list of Platinum with other atoms in the Pt binding site number 1 of X-Ray Structure of the Adduct Between Hen Egg White Lysozyme and Cisplatin at Long Incubation Times within 5.0Å range: probe atom residue distance (Å) B Occ A:Pt203 b:52.1 occ:0.50 PT1 A:CPT203 0.0 52.1 0.5 N1 A:CPT203 2.0 41.4 0.5 N2 A:CPT203 2.1 43.7 0.5 CL2 A:CPT203 2.3 41.5 0.5 ND1 A:HIS15 2.4 47.0 1.0 CG A:HIS15 3.3 33.9 1.0 CE1 A:HIS15 3.4 39.0 1.0 CB A:HIS15 3.5 30.5 1.0 CA A:HIS15 3.6 26.1 1.0 O A:HIS15 4.0 28.7 1.0 C A:HIS15 4.2 24.0 1.0 CD2 A:HIS15 4.5 36.0 1.0 NE2 A:HIS15 4.5 40.6 1.0 O A:ARG14 4.7 26.3 1.0 N A:HIS15 4.7 26.3 1.0 CG2 A:THR89 4.8 30.7 1.0 O A:HOH352 4.9 48.6 1.0 O A:HOH361 4.9 43.1 1.0 OG1 A:THR89 5.0 26.3 1.0

Platinum binding site 2 out of 2 in the X-Ray Structure of the Adduct Between Hen Egg White Lysozyme and Cisplatin at Long Incubation Times


Mono view


Stereo pair view

A full contact list of Platinum with other atoms in the Pt binding site number 2 of X-Ray Structure of the Adduct Between Hen Egg White Lysozyme and Cisplatin at Long Incubation Times within 5.0Å range: probe atom residue distance (Å) B Occ A:Pt204 b:48.2 occ:0.50 PT1 A:CPT204 0.0 48.2 0.5 N1 A:CPT204 2.1 55.9 0.5 NH1 A:ARG14 2.1 58.4 1.0 CL2 A:CPT204 2.2 45.1 0.5 O A:HOH360 2.2 30.2 0.5 NE2 A:HIS15 2.4 40.6 1.0 O A:HOH323 2.5 40.2 0.5 CE1 A:HIS15 3.0 39.0 1.0 CZ A:ARG14 3.2 57.6 1.0 CD2 A:HIS15 3.5 36.0 1.0 O A:HOH382 3.6 19.9 0.5 NE A:ARG14 3.6 55.2 1.0 ND1 A:HIS15 4.2 47.0 1.0 O A:HOH361 4.3 43.1 1.0 NH2 A:ARG14 4.3 52.1 1.0 OD1 A:ASP87 4.3 29.4 1.0 O A:HOH397 4.4 51.6 1.0 CG A:HIS15 4.4 33.9 1.0 O A:HOH413 4.6 61.9 1.0 OG1 A:THR89 4.6 26.3 1.0

G.Ferraro, A.Pica, I.Russo Krauss, F.Pane, A.Amoresano, A.Merlino. Effect of Temperature on the Interaction of Cisplatin with the Model Protein Hen Egg White Lysozyme. J.Biol.Inorg.Chem. V. 21 433 2016.
ISSN: ESSN 1432-1327
PubMed: 27040953
DOI: 10.1007/S00775-016-1352-0