Platinum in PDB 5mij: X-Ray Structure of Carboplatin-Encapsulated Horse Spleen Apoferritin

The structure of X-Ray Structure of Carboplatin-Encapsulated Horse Spleen Apoferritin, PDB code: 5mij was solved by N.Pontillo, G.Ferraro, J.R.Helliwell, A.Merlino, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	104.89 / 1.49
Space group	F 4 3 2
Cell size a, b, c (Å), α, β, γ (°)	181.670, 181.670, 181.670, 90.00, 90.00, 90.00
R / Rfree (%)	15.8 / 18.1

The structure of X-Ray Structure of Carboplatin-Encapsulated Horse Spleen Apoferritin also contains other interesting chemical elements:

Cadmium	(Cd)	12 atoms
Chlorine	(Cl)	4 atoms

The binding sites of Platinum atom in the X-Ray Structure of Carboplatin-Encapsulated Horse Spleen Apoferritin (pdb code 5mij). This binding sites where shown within 5.0 Angstroms radius around Platinum atom.
In total 4 binding sites of Platinum where determined in the X-Ray Structure of Carboplatin-Encapsulated Horse Spleen Apoferritin, PDB code: 5mij:
Jump to Platinum binding site number: 1; 2; 3; 4;

Platinum binding site 1 out of 4 in the X-Ray Structure of Carboplatin-Encapsulated Horse Spleen Apoferritin


Mono view


Stereo pair view

A full contact list of Platinum with other atoms in the Pt binding site number 1 of X-Ray Structure of Carboplatin-Encapsulated Horse Spleen Apoferritin within 5.0Å range: probe atom residue distance (Å) B Occ A:Pt216 b:46.0 occ:0.30 PT A:PT216 0.0 46.0 0.3 PT A:PT216 2.0 25.4 0.3 NE2 A:HIS132 2.9 32.1 1.0 CD2 A:HIS132 3.6 30.1 1.0 CE1 A:HIS132 4.0 35.0 1.0 CB A:SER131 4.2 29.8 1.0 OD2 A:ASP127 4.3 27.7 1.0 O A:HOH338 4.5 21.1 1.0 OG A:SER131 4.5 35.3 1.0 CG A:HIS132 4.9 29.3 1.0

Platinum binding site 2 out of 4 in the X-Ray Structure of Carboplatin-Encapsulated Horse Spleen Apoferritin


Mono view


Stereo pair view

A full contact list of Platinum with other atoms in the Pt binding site number 2 of X-Ray Structure of Carboplatin-Encapsulated Horse Spleen Apoferritin within 5.0Å range: probe atom residue distance (Å) B Occ A:Pt216 b:25.4 occ:0.30 PT A:PT216 0.0 25.4 0.3 PT A:PT216 2.0 46.0 0.3 NE2 A:HIS132 2.2 32.1 1.0 CE1 A:HIS132 3.0 35.0 1.0 CD2 A:HIS132 3.4 30.1 1.0 O A:HOH459 3.7 43.5 1.0 ND1 A:HIS132 4.2 33.5 1.0 CG A:HIS132 4.4 29.3 1.0 NH1 A:ARG64 4.8 43.0 1.0

Platinum binding site 3 out of 4 in the X-Ray Structure of Carboplatin-Encapsulated Horse Spleen Apoferritin


Mono view


Stereo pair view

A full contact list of Platinum with other atoms in the Pt binding site number 3 of X-Ray Structure of Carboplatin-Encapsulated Horse Spleen Apoferritin within 5.0Å range: probe atom residue distance (Å) B Occ A:Pt217 b:42.0 occ:0.30 OE1 A:GLU53 2.2 23.9 0.4 NE2 A:HIS49 2.2 17.8 0.5 CD2 A:HIS49 3.1 19.6 0.5 CE1 A:HIS49 3.2 17.2 0.5 CD A:GLU53 3.4 15.5 0.4 OE2 A:GLU53 4.2 17.4 0.4 ND1 A:HIS49 4.3 21.9 0.5 CG A:HIS49 4.3 16.6 0.5 CG A:GLU53 4.3 14.9 0.4 CG A:GLU53 4.5 15.2 0.6 CD A:GLU53 4.7 19.5 0.6 OE2 A:GLU53 4.7 30.8 0.6

Platinum binding site 4 out of 4 in the X-Ray Structure of Carboplatin-Encapsulated Horse Spleen Apoferritin


Mono view


Stereo pair view

A full contact list of Platinum with other atoms in the Pt binding site number 4 of X-Ray Structure of Carboplatin-Encapsulated Horse Spleen Apoferritin within 5.0Å range: probe atom residue distance (Å) B Occ A:Pt217 b:43.4 occ:0.30 OE2 A:GLU45 2.2 38.6 1.0 NE2 A:HIS49 2.2 19.9 0.5 CE1 A:HIS49 3.0 23.5 0.5 CD A:GLU45 3.2 30.6 1.0 CD2 A:HIS49 3.4 22.8 0.5 CG A:GLU45 3.7 22.4 1.0 CD A:CD210 3.9 61.4 0.3 O A:HOH533 4.2 22.4 0.3 ND1 A:HIS49 4.2 22.4 0.5 OE1 A:GLU45 4.2 26.4 1.0 CG A:HIS49 4.4 18.1 0.5 O A:HOH471 4.7 41.5 1.0 O A:HOH523 4.7 43.4 1.0

N.Pontillo, G.Ferraro, J.R.Helliwell, A.Amoresano, A.Merlino. X-Ray Structure of the Carboplatin-Loaded Apo-Ferritin Nanocage. Acs Med Chem Lett V. 8 433 2017.
ISSN: ISSN 1948-5875
PubMed: 28435532
DOI: 10.1021/ACSMEDCHEMLETT.7B00025