Platinum in PDB 5t7l: Pt(II)-Mediated Copper-Dependent Interactions Between ATOX1 and MNK1

All present enzymatic activity of Pt(II)-Mediated Copper-Dependent Interactions Between ATOX1 and MNK1:
3.6.3.54;

The structure of Pt(II)-Mediated Copper-Dependent Interactions Between ATOX1 and MNK1, PDB code: 5t7l was solved by R.Caliandro, V.Mirabelli, R.Caliandro, A.Rosato, A.Lasorsa, A.Galliani, F.Arnesano, G.Natile, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	41.49 / 2.83
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	47.617, 54.659, 63.719, 90.00, 90.00, 90.00
R / Rfree (%)	20 / 27.3

The structure of Pt(II)-Mediated Copper-Dependent Interactions Between ATOX1 and MNK1 also contains other interesting chemical elements:

Copper

(Cu)

2 atoms

The binding sites of Platinum atom in the Pt(II)-Mediated Copper-Dependent Interactions Between ATOX1 and MNK1 (pdb code 5t7l). This binding sites where shown within 5.0 Angstroms radius around Platinum atom.
In total 2 binding sites of Platinum where determined in the Pt(II)-Mediated Copper-Dependent Interactions Between ATOX1 and MNK1, PDB code: 5t7l:
Jump to Platinum binding site number: 1; 2;

Platinum binding site 1 out of 2 in the Pt(II)-Mediated Copper-Dependent Interactions Between ATOX1 and MNK1


Mono view


Stereo pair view

A full contact list of Platinum with other atoms in the Pt binding site number 1 of Pt(II)-Mediated Copper-Dependent Interactions Between ATOX1 and MNK1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Pt101 b:93.1 occ:0.72 SG B:CYS15 2.3 68.0 0.7 CB A:CYS12 2.4 18.4 0.3 CB A:CYS12 2.7 47.5 0.7 CB B:CYS15 2.7 58.8 0.3 CB B:CYS15 2.7 62.7 0.7 SG A:CYS12 2.8 62.0 0.7 SG B:CYS15 3.1 56.6 0.3 CA A:CYS12 3.5 49.5 0.7 CA A:CYS12 3.5 35.7 0.3 SG A:CYS12 3.6 8.4 0.3 CU A:CU104 3.9 22.7 0.3 CA B:CYS15 4.2 62.8 0.3 CA B:CYS15 4.2 61.7 0.7 N A:CYS12 4.3 47.5 0.3 N A:CYS12 4.3 56.0 0.7 N B:CYS15 4.6 68.5 0.3 N B:CYS15 4.6 68.8 0.7 C A:CYS12 4.6 42.2 0.3 C A:CYS12 4.6 48.7 0.7 N A:GLY13 4.7 48.4 1.0 OG1 B:THR14 4.8 81.8 1.0 OG B:SER17 4.8 92.2 1.0 CG2 A:THR11 4.8 53.9 1.0 O A:THR11 4.9 50.9 1.0 C A:THR11 4.9 56.3 1.0

Platinum binding site 2 out of 2 in the Pt(II)-Mediated Copper-Dependent Interactions Between ATOX1 and MNK1


Mono view


Stereo pair view

A full contact list of Platinum with other atoms in the Pt binding site number 2 of Pt(II)-Mediated Copper-Dependent Interactions Between ATOX1 and MNK1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Pt103 b:0.0 occ:0.36 CE A:LYS3 2.3 90.3 1.0 NZ A:LYS3 2.4 86.5 1.0 O A:HOH202 2.5 43.6 1.0 SG A:CYS41 2.7 59.9 1.0 CD A:LYS3 2.8 88.2 1.0 O A:HOH204 3.1 31.8 0.9 CG A:LYS3 3.7 80.8 1.0 CB A:CYS41 3.7 53.7 1.0 CB A:LYS3 4.8 75.7 1.0 OE2 A:GLU5 4.9 97.4 1.0

A.Lasorsa, M.I.Nardella, A.Rosato, V.Mirabelli, R.Caliandro, R.Caliandro, G.Natile, F.Arnesano. Mechanistic and Structural Basis For Inhibition of Copper Trafficking By Platinum Anticancer Drugs. J.Am.Chem.Soc. V. 141 12109 2019.
ISSN: ESSN 1520-5126
PubMed: 31283225
DOI: 10.1021/JACS.9B05550