Platinum in PDB 2r8j: Structure of the Eukaryotic Dna Polymerase Eta in Complex with 1,2- D(Gpg)-Cisplatin Containing Dna

All present enzymatic activity of Structure of the Eukaryotic Dna Polymerase Eta in Complex with 1,2- D(Gpg)-Cisplatin Containing Dna:
2.7.7.7;

The structure of Structure of the Eukaryotic Dna Polymerase Eta in Complex with 1,2- D(Gpg)-Cisplatin Containing Dna, PDB code: 2r8j was solved by T.Carell, A.Alt, K.Lammens, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 3.10
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	104.090, 104.090, 293.050, 90.00, 90.00, 90.00
R / Rfree (%)	20.8 / 25.7

The structure of Structure of the Eukaryotic Dna Polymerase Eta in Complex with 1,2- D(Gpg)-Cisplatin Containing Dna also contains other interesting chemical elements:

Calcium

(Ca)

4 atoms

The binding sites of Platinum atom in the Structure of the Eukaryotic Dna Polymerase Eta in Complex with 1,2- D(Gpg)-Cisplatin Containing Dna (pdb code 2r8j). This binding sites where shown within 5.0 Angstroms radius around Platinum atom.
In total 2 binding sites of Platinum where determined in the Structure of the Eukaryotic Dna Polymerase Eta in Complex with 1,2- D(Gpg)-Cisplatin Containing Dna, PDB code: 2r8j:
Jump to Platinum binding site number: 1; 2;

Platinum binding site 1 out of 2 in the Structure of the Eukaryotic Dna Polymerase Eta in Complex with 1,2- D(Gpg)-Cisplatin Containing Dna


Mono view


Stereo pair view

A full contact list of Platinum with other atoms in the Pt binding site number 1 of Structure of the Eukaryotic Dna Polymerase Eta in Complex with 1,2- D(Gpg)-Cisplatin Containing Dna within 5.0Å range: probe atom residue distance (Å) B Occ U:Pt45 b:42.1 occ:1.00 PT1 U:CPT45 0.0 42.1 1.0 N7 U:DG4 2.0 44.4 1.0 N2 U:CPT45 2.0 39.4 1.0 N1 U:CPT45 2.0 38.8 1.0 N7 U:DG5 2.0 39.9 1.0 C8 U:DG4 2.9 46.7 1.0 C8 U:DG5 3.0 40.8 1.0 C5 U:DG4 3.0 50.3 1.0 C5 U:DG5 3.0 39.0 1.0 O6 U:DG5 3.2 35.5 1.0 O6 U:DG4 3.4 55.1 1.0 C6 U:DG5 3.5 36.8 1.0 C6 U:DG4 3.6 52.5 1.0 N4 U:DC6 3.8 49.7 1.0 N9 U:DG4 4.1 50.9 1.0 N9 U:DG5 4.2 42.7 1.0 C4 U:DG4 4.2 50.4 1.0 C4 U:DG5 4.2 39.8 1.0 C4 U:DC6 4.3 48.6 1.0 N3 U:DC6 4.4 48.7 1.0 OP2 U:DG4 4.6 67.8 1.0 SD A:MET74 4.8 30.8 1.0 N1 U:DG5 4.9 35.7 1.0 O6 Q:DG13 5.0 26.6 1.0 N1 U:DG4 5.0 54.4 1.0

Platinum binding site 2 out of 2 in the Structure of the Eukaryotic Dna Polymerase Eta in Complex with 1,2- D(Gpg)-Cisplatin Containing Dna


Mono view


Stereo pair view

A full contact list of Platinum with other atoms in the Pt binding site number 2 of Structure of the Eukaryotic Dna Polymerase Eta in Complex with 1,2- D(Gpg)-Cisplatin Containing Dna within 5.0Å range: probe atom residue distance (Å) B Occ T:Pt45 b:50.0 occ:1.00 PT1 T:CPT45 0.0 50.0 1.0 N7 T:DG4 2.0 65.0 1.0 N2 T:CPT45 2.0 57.1 1.0 N7 T:DG5 2.0 64.8 1.0 N1 T:CPT45 2.0 55.1 1.0 C8 T:DG4 2.9 70.5 1.0 C8 T:DG5 3.0 68.3 1.0 C5 T:DG4 3.0 71.8 1.0 C5 T:DG5 3.0 70.4 1.0 O6 T:DG5 3.3 74.2 1.0 O6 T:DG4 3.3 78.1 1.0 C6 T:DG4 3.5 75.3 1.0 C6 T:DG5 3.5 73.3 1.0 N9 T:DG4 4.2 76.2 1.0 C4 T:DG4 4.2 74.9 1.0 N9 T:DG5 4.2 73.6 1.0 C4 T:DG5 4.2 72.6 1.0 OP1 T:DG4 4.2 93.5 1.0 N4 T:DC6 4.4 68.0 1.0 C4 T:DC6 4.8 69.1 1.0 N1 T:DG4 4.9 78.3 1.0 N1 T:DG5 4.9 75.4 1.0

A.Alt, K.Lammens, C.Chiocchini, A.Lammens, J.C.Pieck, D.Kuch, K.P.Hopfner, T.Carell. Bypass of Dna Lesions Generated During Anticancer Treatment with Cisplatin By Dna Polymerase Eta Science V. 318 967 2007.
ISSN: ISSN 0036-8075
PubMed: 17991862
DOI: 10.1126/SCIENCE.1148242